2025 Dynamics and Evolution of Human Viruses Meeting

We just showcased our latest results using our single-cell multi-omic (DNA+PROTEIN) workflow! By staining peripheral blood mononuclear cells from SIV-infected macaques with select oligonucleotide-conjugated antibodies prior to single-cell DNA sequencing, we were able to classify cells as T cells, monocytes, or undetermined. We then evaluated the type of viral genome defects in each cell type, finding that hypermutant genomes were not present in monocytes and, conversely, genomes with deletions at the 5' and 3 ends were only present in T cells. Results beg the question as to how cell type might influence the persistence of specific viral genome defects!